@article{10498,
  keywords = {Animals, Epithelium, Fluorescein-5-isothiocyanate, Fluoresceins, Granuloma, Guinea Pigs, Haptens, Hypersensitivity, Delayed, Immunity, Cellular, leprosy, Lymph Nodes, Mycobacterium leprae, Phagocytes, Thiocyanates, Tuberculin},
  author = {Verghese S and Curtis J and Turk J L},
  title = {Epithelioid cell granuloma induction in the guinea pig by haptenated Mycobacterium leprae.},
  abstract = {<p>Fluorescein isothiocyanate (FITC)-conjugated Mycobacterium leprae (FITC-M. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. At 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with FITC-M. leprae. At 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with haptenated M. leprae compared with those injected with unconjugated M. leprae. At 5 weeks, there was also a significant increase in delayed type hypersensitivity responses to 25 micrograms purified protein derivative. Histologically, epithelioid cell granulomas were seen in these lymph nodes as early as 2 weeks when FITC-M. leprae was used as the source of antigen. Enhancement in the immunogenicity of M. leprae by conjugation with FITC has been postulated.</p>},
  year = {1987},
  journal = {Cellular immunology},
  volume = {107},
  pages = {307-16},
  month = {1987 Jul},
  issn = {0008-8749},
  doi = {10.1016/0008-8749(87)90239-5},
  language = {eng},
}