@article{15252,
  keywords = {Antigens, Bacterial, Chemical Phenomena, Chemistry, Cross Reactions, Disaccharides, Enzyme-Linked Immunosorbent Assay, Epitopes, Glycolipids, Haptens, Humans, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, leprosy, Mycobacterium leprae, Serum Albumin, Bovine, Tuberculosis},
  author = {Cho S N and Fujiwara T and Hunter S W and Rea T H and Gelber R H and Brennan P J},
  title = {Use of an artificial antigen containing the 3,6-di-O-methyl-beta-D-glucopyranosyl epitope for the serodiagnosis of leprosy.},
  abstract = {<p>The coupling of synthetic 3,6-di-O-methyl-beta-D-glucopyranosyl-(1----4)-2,3-di-O-methyl-alpha-L -rhamnopyranose, the hapten determinant of phenolic glycolipid I from Mycobacterium leprae, to bovine serum albumin (BSA) by reductive amination produced the antigen epsilon-N-1-[1-deoxy-2,3-di-O-methyl-4-O-(3',6'-di-O-methyl-beta -D-glucopyranosyl)-rhamnitol]-lysyl-BSA, which proved highly sensitive in ELISA and showed good concordance with the native glycolipid in analysis of serum samples from 223 leprosy patients. Conjugates prepared from 6-O-methyl-beta-D-glucopyranosyl- or beta-D-glucopyranosyl-containing disaccharides were inactive and those containing noncyclic 3,6-di-O-methyl-glucitol showed little activity. Thus 3,6-di-O-methyl-beta-D-glucopyranose in its cyclic hemiacetal form is necessary for binding anti-glycolipid IgM from leprosy patients. Analysis of serum samples from healthy subjects showed a false-positive rate of 2.4% (four of 169) against the glycolipid and 3.6% (six of 169) against the glycoconjugate. Comparable figures for samples of sera of tuberculosis patients were 3.0% (two of 66) and 9.0% (six of 66), respectively. Alternative synthesizing strategies may diminish this cross-reactivity. The prospects of a fully synthetic specific antigen for the worldwide serodiagnosis of leprosy look promising.</p>},
  year = {1984},
  journal = {The Journal of infectious diseases},
  volume = {150},
  pages = {311-22},
  month = {1984 Sep},
  issn = {0022-1899},
  doi = {10.1093/infdis/150.3.311},
  language = {eng},
}