@article{19527,
  keywords = {Adult, Aged, Aged, 80 and over, Antibodies, Bacterial, Antigens, Bacterial, Chaperonin 10, Cross Reactions, Humans, leprosy, Middle Aged, Mycobacterium leprae, Sensitivity and Specificity, Tuberculosis},
  author = {Rojas R E and Demichelis S O and Gimenez M F and Molinari M L and Segal-Eiras A},
  title = {Cross-reactivity of anti-10kD heat shock protein antibodies in leprosy and tuberculosis patients.},
  abstract = {<p>The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed: within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.</p>},
  year = {1997},
  journal = {Medicina},
  volume = {57},
  pages = {581-6},
  month = {1997},
  issn = {0025-7680},
  language = {eng},
}