@article{23107,
  keywords = {Animals, Cattle, Cells, Cultured, Cytokines, Dose-Response Relationship, Immunologic, Fetal Blood, Hot Temperature, Humans, Interleukin 1 Receptor Antagonist Protein, Interleukin-1, Interleukin-6, Kinetics, Monocytes, Mycobacterium bovis, Mycobacterium leprae, Phagocytosis, Sialoglycoproteins, Tumor Necrosis Factor-alpha},
  author = {Suzuki K and Fukutomi Y and Matsuoka M and Torii K and Hayashi H and Takii T and Oomoto Y and Onozaki K},
  title = {Differential production of interleukin 1 (IL-1), IL-6, tumor necrosis factor, and IL-1 receptor antagonist by human monocytes stimulated with Mycobacterium leprae and M. bovis BCG.},
  abstract = {<p>Human blood monocytes cultured in various serum conditions were stimulated with Mycobacterium leprae or M. bovis BCG and their cytokine-inducing abilities were compared. BCG, either live or killed, induced production of interleukin 1 (IL-1), IL-6, tumor necrosis factor (TNF), and IL-1 receptor antagonist (IL-1ra). Live BCG at a lower bacterial number was more potent than killed BCG in the induction of IL-6 and TNF. In contrast to BCG, killed M. leprae induced few cytokines except for IL-1ra. Similar results were obtained when monocytes were cultured in the presence of untreated or heat-inactivated fetal bovine serum (FBS). When FBS and human serum (HS) were compared and the effect of heat inactivation was investigated, monocytes in HS produced the most cytokines, then those in FBS, irrespective of heat inactivation, and those in heat-inactivated HS produced the least cytokines. There were no differences between live and killed M. leprae, and BCG were far more potent than M. leprae in all of our experimental conditions, indicating that the poor cytokine (IL-1, IL-6 and TNF)-inducing ability of M. leprae was not due to their viability. Cytokine production was partially in parallel with the phagocytosis of the mycobacteria. These results suggest that M. leprae favor their infection by evoking little host reaction through the induction of only low levels of immunostimulatory or proinflammatory cytokines but a substantial amount of immunosuppressive cytokine.</p>
},
  year = {1993},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {61},
  pages = {609-18},
  month = {1993 Dec},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v61n4a13.pdf},
  language = {eng},
}