@article{7724,
  keywords = {Agglutination Tests, Antibodies, Bacterial, Antigens, Bacterial, Blood Specimen Collection, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, leprosy, Mycobacterium leprae, Paper, Sensitivity and Specificity},
  author = {Chanteau S and Cartel J L and Boutin J P and Roux J},
  title = {Evaluation of gelatin particle agglutination assay for the detection of anti-PGLI antibodies. Comparison with ELISA method and applicability on a large scale study using blood collected on filter paper.},
  abstract = {<p>Given the technical difficulties of the ELISA method, a gelatin particle agglutination test (MLPA) has been developed recently for the detection of anti-PGLI antibodies. The purpose of this study was to compare these 2 tests. MLPA was found to be less specific than ELISA (91% versus 98%, chi 2 = 66.8, p less than 0.001). The sensitivity of both tests was of 95% for the diagnosis of multibacillary patients. In the case of paucibacillary patients. MLPA was found to be less sensitive than ELISA (21% versus 35%, chi 2 = 6.98, p greater than 0.01). The agreement between the 2 tests for a positive or a negative result was satisfying (85% to 100%), except for the weakly seropositive individuals (71%). The correlation between OD obtained with ELISA and antibody titre obtained with MLPA was statistically significant (r = 0.70, p less than 0.001). Conversely to ELISA, MLPA was not applicable on blood samples absorbed on filter paper without a serious loss of sensitivity. In conclusion, this study demonstrated that the MLPA test can only reliably detect anti-PGLI antibodies in multibacillary cases.</p>},
  year = {1991},
  journal = {Leprosy review},
  volume = {62},
  pages = {255-61},
  month = {1991 Sep},
  issn = {0305-7518},
  url = {http://leprev.ilsl.br/pdfs/1991/v62n3/pdf/v62n3a02.pdf},
  doi = {10.5935/0305-7518.19910030},
  language = {eng},
}