@article{93856,
  keywords = {diagnosis, In-house PCR, leprosy, Metagenomic next-generation sequencing, Mycobacterium leprae},
  author = {Quan M and Liu L and Zhou T and Jiang Y and Wang X and Zong Z},
  title = {Leprosy in a low-incidence setting : Case report relevant to metagenomic next generation sequencing applications.},
  abstract = {<p>Leprosy is a disease caused by Mycobacterium leprae that results in disability. In 2000 the World Health Organization announced that leprosy had been eradicated. In nonendemic areas diagnosing leprosy is becoming a challenge for inexperienced clinicians. This case involves a male patient suffering from chronic numbness, hand deformity and recurrent erythema. Skin biopsy revealed granuloma and acid-fast staining of short-rod bacteria. Peripheral venous blood was subjected to metagenomic next generation sequencing and bioinformatics analysis, which revealed 3 unique sequence reads of M. leprae. Paraffin-embedded tissue and fresh samples scraped from skin lesions were subjected to in-house PCR targeting 16S rRNA, hsp65, rpoB, rpoT, ribF-rpsO, and mmaA. Sanger sequencing of amplicons from fresh samples and paraffin-embedded tissue verified the presence of M. leprae. For inexperienced clinicians in nonendemic areas nucleic acid amplification tests, such as in-house PCR, are helpful for diagnosing leprosy but sequence reads from metagenomic next generation sequencing may also provide evidence when interpreted cautiously.</p>},
  year = {2020},
  journal = {Wiener klinische Wochenschrift},
  month = {04/2020},
  issn = {1613-7671},
  doi = {10.1007/s00508-020-01644-7},
  language = {eng},
}