01748nas a2200169   4500000000100000008004100001260001200042653001500054653001500069100001800084245007700102856004100179300001000220490000700230520132700237022001401564       1967                            d  c01/196710atratamento10aMetabolism1 aPrabhakaran K00aMetabolism of Mycobacterium leprae separated from human leprosy nodules.  uhttp://ila.ilsl.br/pdfs/v35n1a05.pdf  a34-410 v353 a<p>This paper deals with studies on a number of oxidative enzymes in Mycobacterium leprae separated directly from human leprosy nodules. In an investigation such as this, certain limitations are inherent: ( 1 ) the microorganisms have to be separated from the infected tissue in pure form suitable for metabolic studies; (2) only those enzymes can be studied for which technics applicable to small quantities or material are available. Study of M. lepramnurium has become a model system for investigations relating to human leprosy. Since these organisms have also not been cultivated in inert media, they are isolated directly from infected tissues of rats or mice. Gray (9), and Hanks (10), working with whole cells of M. lepraemurium separated from testicular lepromas of rats, could not obtain utilization of 50 different substrates, including various tissue extracts. Ito and Sonoda (.17) demonstrated increase in oxygen-uptake of M. lepraemul'ium with heated extracts of rat testes and testicular lepromas. In cellnfree extracts of M. lepraemurium, Kusaka (18) observed succinate oxidase and glucose-6-phosphate dehydrogenase, which were absent from the whole cells. Mori et al. (19) demonstrated diaphorase I and II and malate dehydrogenase in extracts prepared from lyophilized cells of M. le}J - raemul'ium.</p>
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