02160nas a2200289   4500000000100000008004100001260001200042653002600054653001400080653001500094653002000109100001200129700001300141700001300154700001200167700001100179700001100190700001300201700001100214700001500225245012700240856008700367300000900454490000700463520138600470022001401856       2024                            d  c11/202410aMycobacterium marinum10aEvolution10aPan genome10aVirulence genes1 aZhang M1 aAdroub S1 aUmmels R1 aAsaad M1 aSong L1 aPain A1 aBitter W1 aGuan Q1 aAbdallah A00aComprehensive pan-genome analysis of Mycobacterium marinum: insights into genomic diversity, evolution, and pathogenicity.  uhttps://pmc.ncbi.nlm.nih.gov/articles/PMC11557581/pdf/41598_2024_Article_75228.pdf  a1-120 v143 a<p>Mycobacteria is a diverse genus that includes both innocuous environmental species and serious pathogens like Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium ulcerans, the causative agents of tuberculosis, leprosy, and Buruli ulcer, respectively. This study focuses on Mycobacterium marinum, a closely related species known for its larger genome and ability to infect ectothermic species and cooler human extremities. Utilizing whole-genome sequencing, we conducted a comprehensive pan-genome analysis of 100&nbsp;M. marinum strains, exploring genetic diversity and its impact on pathogenesis and host specificity. Our findings highlight significant genomic diversity, with clear distinctions in core, dispensable, and unique genes among the isolates. Phylogenetic analysis revealed a broad distribution of genetic lineages, challenging previous classifications into distinct clusters. Additionally, we examined the synteny and diversity of the virulence factor CpnT, noting a wide range of C-terminal domain variations across strains, which points to potential adaptations in pathogenic mechanisms. This study enhances our understanding of M. marinum's genomic architecture and its evolutionary relationship with other mycobacterial pathogens, providing insights that could inform disease control strategies for M. tuberculosis and other mycobacteria.</p>
  a2045-2322