01511nas a2200325   4500000000100000008004100001260001300042653001200055653001600067653002300083653001300106653002000119653002300139653002800162653002900190653001200219653001600231653000900247653002400256653002500280653002500305100001600330700001500346700002100361245005900382300001000441490000700451520071300458022001401171       1978                            d  c1978 Oct10aAnimals10aBCG Vaccine10aBacterial Vaccines10aFreezing10aHot Temperature10aImmunity, Cellular10aInjections, Intradermal10aInjections, Subcutaneous10aleprosy10aLymph Nodes10aMice10aMycobacterium bovis10aMycobacterium leprae10aVaccines, Attenuated1 aShepard C C1 aWalker L L1 aVan Landingham R00aHeat stability of Mycobacterium leprae immunogenicity.  a87-930 v223 a<p>The protection provided to mice by vaccines administered intradermally was measured after footpad challenge with Mycobacterium leprae. The protection offered by M. leprae suspensions was not decreased when the vaccines were killed by 60 degrees C heat or at the higher temperatures tested, which included 215 degrees C (autoclave). Even highly purified suspensions retained their immunogenicity. In contrast, the vaccine protection provided by intradermal M. bovis (strain BCG) was markedly reduced when heated to 60 degrees C. The enlargement of the lymph nodes regional to the intradermal vaccines was measured and found generally to parallel the vaccine protection provided by M. leprae and by BCG.</p>  a0019-9567