01386nas a2200313   4500000000100000008004100001260001300042653002000055653002000075653001900095653001400114653001200128653001600140653002500156653001400181653002500195653002600220653001400246653001200260100001500272700001700287700001400304245019300318856007800511300001100589490000600600520045200606022001401058       1974                            d  c1974 Jan10aAutoradiography10aCells, Cultured10aDNA, Bacterial10aInfection10aleprosy10aMacrophages10aMicroscopy, Electron10aMonocytes10aMycobacterium leprae10aStaining and Labeling10aThymidine10aTritium1 aTalwar G P1 aKrishnan A D1 aGupta P D00aQuantitative evaluation of the progress of intracellular infection in vitro: incorporation of 3H-thymidine into deoxyribonucleic acid by Mycobacterium leprae in cultivated blood monocytes.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC414783/pdf/iai00241-0199.pdf  a187-910 v93 a<p>Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-(3)H]thymidine into deoxyribonucleic acid of the bacterial cells. The radioactive precursor is not taken up by the host cells, and evidence has been presented for its incorporation into bacteria. The procedure is sensitive, reproducible, and highly quantitative.</p>  a0019-9567