01812nas a2200313   4500000000100000008004100001260001700042653002200059653001100081653003800092653003200130653003500162653001900197653002700216653001100243653003200254100002100286700001400307700002400321700001500345700002200360700001700382700002100399245013300420300001100553490000700564520091300571022001401484       1990                            d  c1990 Mar-Apr10aBlotting, Western10aBrazil10aEnzyme-Linked Immunosorbent Assay10aEvaluation Studies as Topic10aFluorescent Antibody Technique10aHIV Antibodies10aHemagglutination Tests10aHumans10aSensitivity and Specificity1 aIvo-Dos-Santos J1 aMello D L1 aCouto-Fernandez J C1 aPassos R M1 aDias-Carneiro L A1 aCastilho E A1 aGalvão-Castro B00aEvaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera.  a96-1000 v323 a<p>Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.</p>  a0036-4665