01078nas a2200229 4500000000100000008004100001260001300042653001200055653003100067653001100098653001200109653001800121653003200139653001700171100001100188700002100199245004900220300001000269490000700279520054800286022001400834 1979 d c1979 Sep10aAnimals10aChromatography, Thin Layer10aHumans10aleprosy10aMycolic Acids10aSpectrophotometry, Infrared10aTuberculosis1 aDevi S1 aStewart-Tull E S00aDetection of mycolic acid in leprous tissue. a484-60 v473 a

A method is described for the extraction of mycolic acid from lepromatous leprosy tissues and from tuberculous tissues and its identification by thin layer chromatography. Verification of the mycolic acid extracted and separated by thin layer chromatography was accomplished by infrared spectroscopy. Although the presence of mycolic acid in tissues is not, in itself, diagnostic for leprosy, its demonstration in biopsy tissues might be a useful additional qualitative test if used in conjunction with clinical evidence of the disease.

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