01802nas a2200337   4500000000100000008004100001260001300042653002700055653001100082653001100093653002800104653001200132653001800144653002200162653002100184653001400205653002000219653000900239653001800248653001900266653001800285100001500303700001500318700001500333700001200348245006100360300001100421490000700432520101100439022001401450       1983                            d  c1983 Jun10aAntibodies, Monoclonal10aBiopsy10aHumans10aImmunoenzyme Techniques10aleprosy10aLichen Planus10aMycosis Fungoides10aNevus, Pigmented10aPhenotype10aSarcoma, Kaposi10aSkin10aSkin Diseases10aSkin Neoplasms10aT-Lymphocytes1 aTaylor C R1 aHofman F M1 aModlin R L1 aRea T H00aImmunoperoxidase techniques applied to dermatopathology.  a145-630 v103 a<p>Immunoperoxidase techniques provide the pathologist with the capability for staining a wide range of antigens in tissue sections. More than 100 different antigens have been successfully demonstrated in fixed paraffin sections; other antigens can only be visualized in frozen sections. This latter group particularly includes lymphocyte surface antigens detectable by monoclonal antibodies. This review describes the current state of the art and provides several illustrations of the use of monoclonal antibodies for the identification of T-lymphocyte phenotypes in frozen section from cases of leprosy, mycosis fungoides, halo nevus, Kaposi's sarcoma, lichen planus and atopic dermatitis. Technical details and potential applications are discussed. The growing availability of commercial immunostaining kits makes these techniques more accessible to the surgical pathologist; indeed a whole new range of truly specific, special stains are available, as pathologists we must simply learn to use them.</p>  a0303-6987