02195nas a2200373   4500000000100000008004100001260001300042653001500055653001000070653000900080653001800089653001900107653001700126653001900143653001100162653003000173653001200203653002000215653002600235653003500261653000900296653001600305653001500321653003000336653001700366100001300383700001700396700001600413245008100429300001100510490000700521520127900528022001401807       1980                            d  c1980 Aug10aAdolescent10aAdult10aAged10aBlastomycosis10aConcanavalin A10aHistoplasmin10aHistoplasmosis10aHumans10aHypersensitivity, Delayed10aleprosy10aLeukocyte Count10aLymphocyte Activation10aLymphocyte Culture Test, Mixed10aMale10aMiddle Aged10aSkin Tests10aT-Lymphocytes, Regulatory10aTuberculosis1 aArtz R P1 aJacobson R R1 aBullock W E00aDecreased suppressor cell activity in disseminated granulomatous infections.  a343-520 v413 a<p>The effect of granulomatous infections upon the activity of a T lymphocyte subclass in human peripheral blood that can be induced by concanavalin A (Con A) to function in a suppressor mode was studied. Peripheral blood lymphocytes (PBL) from eleven patients with disseminated mycotic or mycobacterial infections or from controls were preincubated with and without Con A, washed and cultured with allogeneic PBL freshly drawn from healthy donors sensitive to histoplasmin. DNA synthesis was then measured in co-cultures stimulated by Con A, histoplasmin, or by the mixed lymphocyte culture (MLC) reaction alone. As compared with cells preincubated without Con A, the Con A-pretreated cells were significantly less effective in suppressing the responses of normal PBL to histoplasmin (P < 0.01), and in a one-way MLC reaction (P < 0.05). The Con A-induced suppressor activity of PBL from nine patients with localized granulomatous infections did not differ significantly from that exerted by PBL of normal controls in two of the three co-culture systems employed. These studies suggest that either dysfunction or a reduction of the Con A-inducible T-suppressor cell subpopulation in peripheral blood is frequent among patients was disseminated granulomatous infections.</p>  a0009-9104