01426nas a2200289 4500000000100000008004100001260001300042653001200055653003100067653001100098653000900109653001100118653001200129653000900141653001800150653001100168653001100179100001200190700001500202700001400217245011300231856004100344300000800385490000700393520072200400022001401122 1980 d c1980 Mar10aAnimals10aBacteriological Techniques10aFemale10aFoot10aHumans10aleprosy10aMice10aMycobacterium10aNorway10aPlants1 aKazda J1 aIrgens L M1 aMüller K00aIsolation of non-cultivable acid-fast bacilli in sphagnum and moss vegetation by foot pad technique in mice. uhttp://ila.ilsl.br/pdfs/v48n1a01.pdf a1-60 v483 a

In the former leprosy-endemic coastal area of Norway, 122 samples of sphagnum and moss vegetation were collected from 6 biotopes and examined for non-cultivable AFB by foot pad inoculation. Of the 759 foot pads examined, 20% contained non-cultivable AFB. A significantly higher frequency was found in a habitat where Sphagnum cuspidatum was preponderant, the sphagnum species from which the maximum yield was obtained. The bacteria were polymorphous, solidly staining AFB, which multiplied in passage in foot pads while they could not be cultivated on the conventional media for mycobacteria. Efforts are continuing to identify these AFB by biochemical methods and by inoculation into nine-banded armadillos.

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