01967nas a2200373   4500000000100000008004100001260001300042653001500055653001000070653000900080653002400089653001600113653002300129653001100152653001100163653001200174653000900186653001600195653003100211653001800242653001700260653001600277100001400293700001600307700001400323700001200337700002100349700001600370245017000386300001200556490000800568520100300576022001401579       1994                            d  c1994 Nov10aAdolescent10aAdult10aAged10aAntigens, Bacterial10aBCG Vaccine10aBacterial Proteins10aFemale10aHumans10aleprosy10aMale10aMiddle Aged10aMycobacterium tuberculosis10aT-Lymphocytes10aTuberculosis10aVaccination1 aRoche P W1 aTriccas J A1 aAvery D T1 aFifis T1 aBillman-Jacobe H1 aBritton W J00aDifferential T cell responses to mycobacteria-secreted proteins distinguish vaccination with bacille Calmette-Guérin from infection with Mycobacterium tuberculosis.  a1326-300 v1703 a<p>The immune responses of healthy recipients of Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccine, tuberculosis (TB) patients, and contacts of TB patients were examined to three major secretory proteins of Mycobacterium tuberculosis, MPB59, MPB64, and MPB70. MPB59 evoked a T cell response in 78% of BCG vaccines, 62% of TB patients, and 60% of contacts. MPB64 and MPB70 were recognized by < 15% of BCG vaccinees, half of TB patients, and three-quarters of contacts. TB and leprosy patients had antibody responses to MPB59, but few had antibodies to MPB64 or MPB70. Hybridization of mycobacterial DNA with specific gene probes demonstrated the absence of a gene for MBP64 in the vaccine strain of BCG, but the MPB70 gene was found in all virulent and vaccine BCG strains tested. Since MPB64 and MPB70 can induce delayed-type hypersensitivity reactions in infected animals, either of these proteins may have potential as skin test reagents for detecting infection with M. tuberculosis.</p>  a0022-1899