01669nas a2200289   4500000000100000008004100001260001300042653002400055653002000079653001100099653001000110653001200120653001700132653001400149653001700163653001600180100001400196700001700210700001200227700001500239700001400254245009600268300001100364490000700375520098300382022001401365       1993                            d  c1993 Sep10aAntigens, Bacterial10aCells, Cultured10aHumans10aLatex10aleprosy10aMicrospheres10aMonocytes10aPhagocytosis10aSuperoxides1 aHasan R S1 aDockrell H M1 aJamil S1 aChiang T J1 aHussain R00aAntigen-coated latex particles as a model system for probing monocyte responses in leprosy.  a3724-90 v613 a<p>To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of MLSON-coated particles was greater than that of control particles in monocytes from both leprosy patients and controls from leprosy-endemic areas; uptake of MLSON-coated particles was higher in monocytes from lepromatous leprosy patients than in cells from tuberculoid leprosy patients and controls. In both patients and controls, uptake of latex particles coated with leprosy antigens triggered very little reduction of nitroblue tetrazolium although the cells were capable of mounting a respiratory burst. Antigen-coated latex particles can therefore be used as a tool to investigate monocyte responses to M. leprae and individual recombinant antigens.</p>  a0019-9567