01806nas a2200337   4500000000100000008004100001260000900042653001000051653000900061653002200070653002600092653002400118653001800142653002000160653001100180653001200191653001600203653002500219653003200244653001700276100001400293700001900307700001600326700001700342700001800359245010200377300001000479490000700489520095800496022001401454       1997                            d  c199710aAdult10aAged10aAged, 80 and over10aAntibodies, Bacterial10aAntigens, Bacterial10aChaperonin 1010aCross Reactions10aHumans10aleprosy10aMiddle Aged10aMycobacterium leprae10aSensitivity and Specificity10aTuberculosis1 aRojas R E1 aDemichelis S O1 aGimenez M F1 aMolinari M L1 aSegal-Eiras A00aCross-reactivity of anti-10kD heat shock protein antibodies in leprosy and tuberculosis patients.  a581-60 v573 a<p>The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed: within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.</p>  a0025-7680