01750nas a2200253   4500000000100000008004100001260001300042653002600055653004200081653001100123653001200134653002100146653002400167653001700191100001200208700001200220700001300232245015600245856004100401300001100442490000700453520102200460022001401482       1998                            d  c1998 Jun10aAntibodies, Bacterial10aElectrophoresis, Gel, Two-Dimensional10aHumans10aleprosy10aMolecular Weight10aMycobacterium bovis10aTuberculosis1 aNaito M1 aIzumi S1 aYamada T00aTwo-dimensional electrophoretic analysis of humoral responses to culture filtrate of Mycobacterium bovis BCG in patients with leprosy and tuberculosis.  uhttp://ila.ilsl.br/pdfs/v66n2a10.pdf  a208-130 v663 a<p>Sera from 3 lepromatous (LL), 3 borderline lepromatous (BL), 3 mid-borderline (BB), 3 borderline tuberculoid (BT), 2 tuberculoid (TT) and 4 tuberculosis (TB) patients and 3 healthy individuals were examined for their reactivities against the proteins in the culture filtrate of BCG separated by two-dimensional electrophoresis (2-DE). The sera were obtained from patients who were untreated. Sera from LL and BL patients reacted strongly with the antigen 85 (Ag85) complex and MPB51. Sera from LL and BL patients also weakly reacted with the newly identified 29-, 24- and 23-kDa spots. Sera from the other patients reacted similarly, but the levels of reaction were remarkably lower than those form LL and BL patients. Mycobacterium leprae antigens that are analogous to BCG AG85 and MPB51 are suggested as the main targets for the humoral immunity of untreated patients. The reactivities of sera with newly identified antigens may provide the potential for predicting the severity and prognosis of diseases.</p>
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