01936nas a2200277   4500000000100000008004100001653001300042653001300055653001200068653001400080653001400094653002300108100001400131700001400145700001500159700001800174700001500192700001300207700001200220245011800232856006700350300001400417490000600431050001700437520120400454       2012                            d10aMutation10aM.leprae10aleprosy10aIndonesia10afolP gene10aDapsone resistance1 aAdriaty D1 aWahyuni R1 aIswahyudi 1 aPrakoeswa CRS1 aAbdullah R1 aAgusni I1 aIzumi S00aDapsone resistance in a Mycobacterium leprae isolate with two point mutations in folP gene from a leprosy patient  uhttp://journal.itd.unair.ac.id/index.php/IJTID/article/view/92  a108 - 1110 v3  aADRIATY 20123 aDrug resistance in leprosy is important for Leprosy Control Program, since the WHO-Multidrug regiment (MDT) has been used
for global treatment of leprosy for more than two decades already. A Dapsone resistance case in a Multibacillary (MB) leprosy case
is reported. The patient was diagnosed and treated in Tajuddin Chalid Hospital Makassar, South Sulawesi. Previously he was treated
in a health center at South Sulawesi and was given a treatment for one year, before referred to the hospital. The leprosy skin lesions
are still active with erythematous skin lesions and thickened ear lobe. Bacteriological examination was positive for Acid Fast Bacilli,
the Bacterial Index was 3+ and the Morphological Index was 1%. The specimens of M.leprae isolation was sent to the Institute of
Tropical Disease Surabaya for drug resistance study. Using the Lp1-2 and Lp3-4 nested primers, PCR test was positive for M.leprae.
Sequencing result for folP gene showed a double mutation at codon 53 (ACC / Threonin ) which become (AGG / Arginine). Simultaneous
mutation at two nucleotides at one codon has never been reported in Indonesia before and this phenomenon is important for leprosy
control policy.