01972nas a2200253   4500000000100000008004100001653001700042653002400059653001500083653001400098653001400112653001200126100001400138700001400152700001600166700001300182700001200195245014400207856006700351300001200418490000600430050001700436520126500453       2010                            d10aTransmission10aMycobacteria leprae10aTTC repeat10aEast Java10aIndonesia10aleprosy1 aAdriaty D1 aWahyuni R1 aIswahyudi I1 aAgusni I1 aIzumi S00aTTC repeats variation of Mycobacterium leprae isolates foraAnalysis of leprosy transmission in leprosy endemic area in East Java, Indonesia  uhttp://journal.itd.unair.ac.id/index.php/IJTID/article/view/18  a38 - 410 v1  aADRIATY 20103 aEast Java province still has some pocket of leprosy endemic areas. In order to solve the problem, molecular typing will make it feasible to study the transmission pattern of Mycobacterium leprae in leprosy endemic area. The present study is to analyze the presence of M.leprae DNA in the environment and to study variation number of TTC repeats and their distribution. Poteran Island is located in Madura, East Java and was chosen because this island has a high prevalence of leprosy and remains stable for the last five years. All samples were analyzed by PCR and the numbers of TTC repeats were confirmed by direct sequencing. Of all collected samples, 26.4% isolates of water resources (24); 61.9%nasal swabs (26); and 35.3% skin tissues (24) are positives. No statistically difference in the pattern distribution of TTC repeats between skin tissues of patients and nasal swab of households contact (p=0.594); also distribution of TTC repeats between skin tissues of leprosy patients and those of water resources (p=0.441); and distribution of TTC repeats between nasal swab of households contact with water resources (p=0.906). It means that the transmission of M.leprae in leprosy endemic area has closely related in 3 aspects: agent, host & environment.