02137nas a2200409   4500000000100000008004100001260001300042653001000055653002600065653002000091653003800111653002900149653001100178653002700189653001100216653002800227653001200255653003200267653001300299653002700312653002300339100000900362700001100371700001100382700001000393700001000403700000900413700000900422700001100431700001100442700001200453245011000465300001100575490000700586520112000593022001401713       2013                            d  c2013 Dec10aAdult10aAntibodies, Bacterial10aCross Reactions10aEnzyme-Linked Immunosorbent Assay10aFalse Positive Reactions10aFemale10aHemagglutination Tests10aHumans10aImmunoenzyme Techniques10aReagins10aSensitivity and Specificity10aSyphilis10aSyphilis Serodiagnosis10aTreponema pallidum1 aGu W1 aYang Y1 aWang Q1 aPan B1 aGuo W1 aWu L1 aHu W1 aYang S1 aSong B1 aZhang C00aComparing the performance of traditional non-treponemal tests on syphilis and non-syphilis serum samples.  a919-250 v243 a<p>The goal of the present study was to determine the performance of two traditional non-treponemal tests for syphilis. Syphilis sera (n = 209) included different stages of disease, and control sera (n = 247) were from patients with tumours, leprosy, systemic lupus erythematosus, hepatitis, pregnant women and healthy individuals. Treponema pallidum ELISA, Treponema pallidum particle agglutination and rapid treponema-specific tests were used as gold standards. Rapid plasma reagin or toluidine red unheated serum test had a sensitivity and specificity of over 95%. False-negative reactions of rapid plasma reagin and toluidine red unheated serum test were observed mainly in primary and latent syphilis cases, and false-positive reactions were present in systemic lupus erythematosus, hepatitis-infected patients. Overall, both non-treponemal tests had high sensitivities and specificities making the assays attractive as screening tests for syphilis. When examined on WHO reference serum samples and based on lower limits of detection, non-treponemal tests were less sensitive than treponema-specific tests.</p>  a1758-1052