02092nas a2200469   4500000000100000008004100001260001300042653001600055653001800071653000900089653003200098653004000130653002300170653002500193653001600218653000900234653002800243653003800271653001100309653001100320653001900331653001000350653002500360653001100385653001900396653000900415653001000424653001500434100001000449700001100459700001200470700001100482700001000493700001200503700000900515245015300524856008000677300001000757490000700767520083400774022001401608       2014                            d  c2014 Mar10aYoung Adult10aSkin Diseases10aSkin10aSensitivity and Specificity10aReal-Time Polymerase Chain Reaction10aParaffin Embedding10aMycobacterium leprae10aMiddle Aged10aMale10aLeprosy, Paucibacillary10aInterspersed Repetitive Sequences10aHumans10aFemale10aDNA, Bacterial10aChild10aCase-Control Studies10aBiopsy10aBacterial Load10aAged10aAdult10aAdolescent1 aYan W1 aXing Y1 aYuan LC1 aYang R1 aTan F1 aZhang Y1 aLi H00aApplication of RLEP real-time PCR for detection of M. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945699/pdf/tropmed-90-524.pdf  a524-90 v903 a<p>The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.</p>
  a1476-1645