01908nas a2200181   4500000000100000008004100001653001200042653002100054653001400075100001200089700001100101700001300112245012000125300001400245490000700259520144700266022001301713       2015                            d10aleprosy10aMedicinal plants10aAyurvedic1 aJani JN1 aJha SK1 aNagar DS00aRoot explant produces multiple shoot from pericycle in Psoralea corylifolia – a leprosy destroyer medicinal plant  a324 - 3290 v673 a<p>Babchi (<em>Psoralea corylifolia</em> L.) is a rare medicinal plant. It has been considered to be efficacious in leprosy and hence given the name ‘Kushtanashini’ (leprosy destroyer) in Ayurveda. The high death rate in wild along with aimless and unlawful gathering and destruction of habitat put the plant in the category of endangered list. In this study a highly reproducible <em>in vitro</em> regeneration system in <em>P. corylifolia</em> using root segments was developed. The effect of different PGRs (Kin BA, and NAA) on the percentage of adventitious shoot induction and the number of shoot buds per root explant was assessed. The maximum response (65.57%) was observed in the media supplemented with 2.22&nbsp;μM BAP and 6.98&nbsp;μM Kin. The treatment had in average 39.67 number of shoot buds and 3.37 elongated shoots. Elongation response was best (92.0%) in MS&nbsp;+&nbsp;2.22&nbsp;μM BAP&nbsp;+&nbsp;6.98&nbsp;μM Kin media fortified with 2.89&nbsp;μM GA<sub>3</sub> and 39.7&nbsp;μM&nbsp;PG, producing a mean of 9.3 elongated shoots with an average of 4.6&nbsp;cm length. An anatomical study showed that the origin of shoot buds were directly from pericycle of root. Elongated shoots were carefully excised and rooted on basal MS&nbsp;+&nbsp;4.92&nbsp;μM IBA&nbsp;+&nbsp;158.6&nbsp;μM&nbsp;PG, with 92 per cent rooting. Rooted explants survived in soil: vermicompost: vermiculite (1:1:1) with 100% success.</p>
  a09266690