01962nas a2200229   4500000000100000008004100001653001200042653001300054653001300067653001000080100001400090700001300104700001600117700001300133700001300146700001200159245017700171300001200348490000700360520135200367022001301719       2015                            d10aleprosy10aCytokine10agenotype10aIndia1 aTarique M1 aNaqvi RA1 aSantosh K V1 aKamal VK1 aKhanna N1 aRao D N00aAssociation of TNF-α-308(GG), IL-10−819(TT), IL-10−1082(GG) and IL-1R1+1970(CC) genotypes with the susceptibility and progression of leprosy in North Indian population  a61 - 650 v733 a<p>Leprosy is an infectious disease caused by <em>M</em>. <em>leprae</em>. We analyzed 48 cytokine polymorphisms in 13 (pro as well as anti-inflammatory) cytokine genes using PCR-SSP assay in 102 leprosy patients and 120 healthy controls with intent to find out a link between cytokine polymorphisms and disease susceptibility. TNF-α (−308) GG, IL-10 (−819) TT, IL-10 (−1082) GG and IL1R (+1970) CC genotypes are found to be predominant (<em>p</em>&nbsp;=&nbsp;0.01, <em>p</em>&nbsp;=&nbsp;0.02, <em>p</em>&nbsp;=&nbsp;0.0001 and <em>p</em>&nbsp;=&nbsp;0.001, respectively) in both tuberculoid as well as lepromatous leprosy patients. This observation suggests these genotypes as play the central role(s) in the progression of disease. CBA assay demonstrates the varied serum concentration of these cytokines with respect to their genotypes. The above genotypes appeared as high producer genotypes in our study. Even in presence of high produce genotypes, TNF-α level are found to be affected/masked by the presence of IL-10 in leprosy patients. Expressional masking of TNF-α is associated with the expression of IL-10 in these patients. This is one the negative impact of SNP–SNP interaction in leprosy patients. Therefore, we can conclude that cytokine gene polymorphisms determine the predisposition to the leprosy progression.</p>
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