03610nas a2200313   4500000000100000008004100001260003700042653001100079653001300090100001700103700001600120700001600136700001400152700001600166700001500182700001400197700001100211700001500222700001100237700001200248700001400260700001400274700001300288245012600301856004700427490000600474520280200480022001403282       2015                            d  bAmercan Society for Microbiology10aGenome10aGenetics1 aTufariello J1 aKerantzas C1 aVilchèze C1 aCalder BR1 aNordberg EK1 aFischer JA1 aHartman T1 aYang E1 aDriscoll T1 aCole L1 aSebra R1 aMaqbool S1 aWattam AR1 aJacobs W00aThe Complete Genome Sequence of the Emerging Pathogen Mycobacterium haemophilum Explains Its Unique Culture Requirements.  uhttp://mbio.asm.org/content/6/6/e01313-15 0 v63 a<p><strong>Editor's abstract: </strong>Mycobacterium haemophilum is an emerging pathogen associated with a variety of clinical syndromes, most commonly skin infections in immunocompromised individuals. M.&nbsp;haemophilum exhibits a unique requirement for iron supplementation to support its growth in culture, but the basis for this property and how it may shape pathogenesis is unclear. Using a combination of Illumina, PacBio, and Sanger sequencing, the complete genome sequence of M.&nbsp;haemophilum was determined. Guided by this sequence, experiments were performed to define the basis for the unique growth requirements of M.&nbsp;haemophilum. We found that M.&nbsp;haemophilum, unlike many other mycobacteria, is unable to synthesize iron-binding siderophores known as mycobactins or to utilize ferri-mycobactins to support growth. These differences correlate with the absence of genes associated with mycobactin synthesis, secretion, and uptake. In agreement with the ability of heme to promote growth, we identified genes encoding heme uptake machinery. Consistent with its propensity to infect the skin, we show at the whole-genome level the genetic closeness of M.&nbsp;haemophilum with Mycobacterium leprae, an organism which cannot be cultivated in vitro, and we identify genes uniquely shared by these organisms. Finally, we identify means to express foreign genes in M.&nbsp;haemophilum. These data explain the unique culture requirements for this important pathogen, provide a foundation upon which the genome sequence can be exploited to improve diagnostics and therapeutics, and suggest use of M.&nbsp;haemophilum as a tool to elucidate functions of genes shared with M.&nbsp;leprae.</p>

<p><strong>IMPORTANCE: </strong>Mycobacterium haemophilum is an emerging pathogen with an unknown natural reservoir that exhibits unique requirements for iron supplementation to grow in vitro. Understanding the basis for this iron requirement is important because it is fundamental to isolation of the organism from clinical samples and environmental sources. Defining the molecular basis for M.&nbsp;haemophilium's growth requirements will also shed new light on mycobacterial strategies to acquire iron and can be exploited to define how differences in such strategies influence pathogenesis. Here, through a combination of sequencing and experimental approaches, we explain the basis for the iron requirement. We further demonstrate the genetic closeness of M.&nbsp;haemophilum and Mycobacterium leprae, the causative agent of leprosy which cannot be cultured in vitro, and we demonstrate methods to genetically manipulate M.&nbsp;haemophilum. These findings pave the way for the use of M.&nbsp;haemophilum as a model to elucidate functions of genes shared with M.&nbsp;leprae.</p>
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