02238nas a2200277   4500000000100000008004100001653001700042653001300059653002300072100001400095700001600109700001700125700001700142700001400159700002100173700001600194700001700210700002000227700001500247700001800262245013700280300001700417490000700434520150500441022001401946       2015                            d10aTransmission10aSerology10aHousehold contacts1 aPinho J D1 aRivas P M S1 aMendes M B P1 aSoares R E P1 aCosta G C1 aNascimento F R F1 aPaiva M F L1 aAquino D M C1 aFigueireido I A1 aSantos A M1 aPereira S R F00aPresence of Mycobacterium leprae DNA and PGL-1 antigen in household contacts of leprosy patients from a hyperendemic area in Brazil.  a14479 -144870 v143 a<p>Editor's abstract:</p>

<p>Leprosy is a highly infectious disease endemic to underdevel-oped countries. In Maranhão State, Northeastern Brazil, the hyperendemic rate of 56.11 cases/100,000 inhabitants increased the necessity of better understanding the epidemiological profile of this population, particularly re-garding efficient methods for evaluating individuals residing with diagnosed patients to understand disease transmission and the risk of infection.</p>

<p>In this study, we examined the percentage of contacts with positive indices for Mycobacterium leprae DNA and phenol-glycolipid-1 antigen (PGL-1). PGL-1 was analyzed by an enzyme-linked immunosorbent assay, the ML-Flow test, and polymerase chain reaction of oral and nasal secretions of 808 leprosy contacts from Maranhão. PGL-1 was detected in 14.0% of pa-tients and differed by operational classification of the index case (P &lt; 0.05). Seropositive results of ML-Flow were 15.0% and identified individuals with and without Bacillus Calmette-Guérin vaccine scars. Molecular diagnosis detected M. leprae DNA in 5.6% of oral samples and 4.6% of nasal tissues, and 87% of subjects resided with high bacillary load patients.</p>

<p>This study reinforces the efficacy of combining molecular and serological techniques to identify potential bacillus carriers in the asymptomatic stage of infection, such as in household contacts, highlighting the importance of these meth-ods for monitoring hyperendemic populations.</p>
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