02287nas a2200289 4500000000100000008004100001653002000042653002400062100001400086700001200100700001800112700001300130700001500143700001000158700001500168700001400183700001500197700001500212700001400227700001300241245006600254856005800320300000800378490000600386520159100392022001401983 2015 d10aPathophysiology10aMolecular diagnosis1 aBelone AF1 aRosa PS1 aTrombone AP F1 aFachin L1 aGuidella C1 aUra S1 aBarreto JA1 aPinilla M1 aCarvalho A1 aCarraro DM1 aSoares FA1 aSoares C00aGenome-Wide Screening of mRNA Expression in Leprosy Patients. uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653304/ a3340 v63 a
Editor's Abstract:
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1580 differentially expressed mRNAs [Fold Change (FC) ≥ 2.0, p ≤ 0.05] in diseased lesions vs. healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type "1" reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type "2" reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.
for free full text version see also: http://journal.frontiersin.org/article/10.3389/fgene.2015.00334/abstract
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