01730nas a2200313   4500000000100000008004100001653001200042653002500054653001400079653002100093653001900114653001300133653001700146653001100163100001300174700001300187700001400200700001900214700001100233700001600244700001500260700001700275700001400292700001300306245012600319300000800445520094900453022001401402       2018                            d10aleprosy10aMycobacterium leprae10aVNTR loci10aCluster Analysis10aGenetic marker10agenotype10aNasal Cavity10aBrazil1 aLima LNC1 aFrota CC1 aSuffys PN1 aBrum Fontes AN1 aMota R1 aAlmeida RLF1 aPontes MAA1 aGonçalves H1 aKendall C1 aKerr LRS00aGenotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.  a1-73 a<p>This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion&nbsp;(NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Ceará. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS.</p>
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