02382nas a2200589   4500000000100000008004100001260001600042653001500058653002100073653002800094653001400122653003000136653003100166653002600197653001100223653002300234653002100257653002500278653002500303653002600328653002700354653003100381653004400412653003000456653003300486653002800519653002700547653002400574653001800598100001600616700000900632700001400641700001400655700001200669700001500681700001400696700001200710700001300722700001900735700001000754700001400764700001400778700001200792700001600804700001400820245008700834300001200921490000800933050001800941520081900959022001401778       2003                            d  c2003 Sep 1210aAlgorithms10aCluster Analysis10aColony Count, Microbial10aCytokines10aGene Expression Profiling10aGene Expression Regulation10aGenes, Immunoglobulin10aHumans10aImmunity, Cellular10aImmunity, Innate10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aMacrophages, Alveolar10aMembrane Glycoproteins10aMycobacterium tuberculosis10aOligonucleotide Array Sequence Analysis10apolymerase chain reaction10aPrincipal Component Analysis10aReceptors, Cell Surface10aReceptors, Immunologic10aToll-Like Receptors10aUp-Regulation1 aBleharski J1 aLi H1 aMeinken C1 aGraeber T1 aOchoa M1 aYamamura M1 aBurdick A1 aSarno E1 aWagner M1 aRöllinghoff M1 aRea T1 aColonna M1 aStenger S1 aBloom B1 aEisenberg D1 aModlin RL00aUse of genetic profiling in leprosy to discriminate clinical forms of the disease.  a1527-300 v301  aBLEHARSKI20033 a<p>Leprosy presents as a clinical and immunological spectrum of disease. With the use of gene expression profiling, we observed that a distinction in gene expression correlates with and accurately classifies the clinical form of the disease. Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated in lesions of lepromatous patients suffering from the disseminated form of the infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production from interleukin-12 toward interleukin-10 and by blocking antimicrobial activity triggered by Toll-like receptors. Gene expression profiles may be useful in defining clinical forms of disease and providing insights into the regulation of immune responses to pathogens.</p>  a1095-9203