01818nas a2200349   4500000000100000008004100001260001300042653002300055653001300078653001100091653001000102653001200112653002500124653001700149653002800166100001400194700001300208700001200221700001000233700001300243700001300256700001500269700001400284700001300298700001300311700001400324245010900338300001100447490000600458520099000464022001401454       2007                            d  c2007 Sep10aBacterial Proteins10agenotype10aHumans10aIndia10aleprosy10aMycobacterium leprae10aSigma Factor10aTandem Repeat Sequences1 aLavania M1 aKatoch K1 aSingh H1 aDas R1 aGupta AK1 aSharma R1 aChauhan DS1 aSharma VD1 aSachan P1 aSachan S1 aKatoch VM00aPredominance of three copies of tandem repeats in rpoT gene of Mycobacterium leprae from Northern India.  a627-310 v73 a<p>This study has been carried out to get understanding of the origin among the strains of Mycobacterium leprae in patients from Northern India by using number of tandem repeats in rpoT gene as marker. Biopsies were collected from hundred leprosy cases (paucibacillary (PB) as well as multibacillary (MB)) across the spectrum from patients attending clinic at JALMA or diagnosed in Field Unit at Ghatampur (Kanpur). These biopsies were homogenized and DNA was extracted by a physiochemical procedure. rpoT region was amplified by using the primers and conditions earlier published. Among 100 strains from Northern Indian patients, 89% exhibited the presence of three copies of the 6bp tandem repeat in the rpoT gene, while 11% contained four copies. These profiles along with other genotyping data may help in studying the historical spread of leprosy by strains of M. leprae disseminated by various human races that migrated to Northern India from other places of Asian continent.</p>  a1567-1348