02078nas a2200397   4500000000100000008004100001260001300042653001100055653002500066653002800091653001100119653002000130653001100150653002100161653002800182653001900210653001200229653000900241653003000250653003600280653003000316653002800346100001500374700001500389700001100404700001300415700001300428700001300441700001600454700001800470245010800488300001200596490000800608520105000616022001401666       2005                            d  c2005 Nov10aBrazil10aCase-Control Studies10aDNA Mutational Analysis10aFemale10aGenetic Markers10aHumans10aImmunity, Innate10aIndians, South American10aInterleukin-1010aleprosy10aMale10apolymerase chain reaction10aPolymorphism, Single Nucleotide10aPromoter Regions, Genetic10aQuantitative Trait Loci1 aMalhotra D1 aDarvishi K1 aSood S1 aSharma S1 aGrover C1 aRelhan V1 aReddy B S N1 aBamezai R N K00aIL-10 promoter single nucleotide polymorphisms are significantly associated with resistance to leprosy.  a295-3000 v1183 a<p>The minor haplotype -3575A/-2849G/-2763C in IL-10 promoter has been defined as a marker of disease resistance to leprosy and its severity in Brazilian population. Our investigation of six single-nucleotide polymorphisms (SNPs) in IL-10 promoter in 282 Indian leprosy patients and 266 healthy controls by direct PCR sequencing, however, showed that the extended haplotype: -3575T/-2849G/-2763C/-1082A/-819C/-592C was associated with resistance to leprosy per se and to the development of severe form of leprosy, using either a binomial (controls vs cases, P=0.01, OR=0.58, CI=0.37-0.89) or ordinal (controls vs paucibacillary vs multibacillary, P=0.004) model. Whereas, IL-10 haplotype -3575T/-2849G/-2763C/-1082A/-819T/-592A was associated with the risk of development of severe form of leprosy (P=0.0002) in contrast to the minor risk haplotype -3575T/-2849A/-2763C in the Brazilian population. The role of IL-10 promoter SNPs in Brazilian and Indian population strongly suggests the involvement of IL-10 locus in the outcome of leprosy.</p>  a0340-6717