02131nas a2200325 4500000000100000008004100001260001300042653002400055653002600079653002400105653003000129653003800159653001600197653001100213653001200224653002500236653001000261653003200271100001500303700001500318700001500333700001100348245020700359856005900566300001100625490000700636050003200643520111600675022001401791 1991 d c1991 Sep10aAgglutination Tests10aAntibodies, Bacterial10aAntigens, Bacterial10aBlood Specimen Collection10aEnzyme-Linked Immunosorbent Assay10aGlycolipids10aHumans10aleprosy10aMycobacterium leprae10aPaper10aSensitivity and Specificity1 aChanteau S1 aCartel J L1 aBoutin J P1 aRoux J00aEvaluation of gelatin particle agglutination assay for the detection of anti-PGLI antibodies. Comparison with ELISA method and applicability on a large scale study using blood collected on filter paper. uhttp://leprev.ilsl.br/pdfs/1991/v62n3/pdf/v62n3a02.pdf a255-610 v62 aInfolep Library - available3 a

Given the technical difficulties of the ELISA method, a gelatin particle agglutination test (MLPA) has been developed recently for the detection of anti-PGLI antibodies. The purpose of this study was to compare these 2 tests. MLPA was found to be less specific than ELISA (91% versus 98%, chi 2 = 66.8, p less than 0.001). The sensitivity of both tests was of 95% for the diagnosis of multibacillary patients. In the case of paucibacillary patients. MLPA was found to be less sensitive than ELISA (21% versus 35%, chi 2 = 6.98, p greater than 0.01). The agreement between the 2 tests for a positive or a negative result was satisfying (85% to 100%), except for the weakly seropositive individuals (71%). The correlation between OD obtained with ELISA and antibody titre obtained with MLPA was statistically significant (r = 0.70, p less than 0.001). Conversely to ELISA, MLPA was not applicable on blood samples absorbed on filter paper without a serious loss of sensitivity. In conclusion, this study demonstrated that the MLPA test can only reliably detect anti-PGLI antibodies in multibacillary cases.

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