01618nas a2200265   4500000000100000008004100001260001200042653001300054653002500067653002000092653001200112653001800124100002500142700001500167700001400182700001600196700001500212700001500227700001400242245013500256300001200391490000700403520092800410022001401338       2020                            d  c01/202010aLF tests10aMycobacterium leprae10aglycoconjugates10aleprosy10aserodiagnosis1 aKorolyova-Ushakova A1 aBaranova E1 aIgnatov S1 aFedyukina G1 aSolov'ev P1 aKolombet L1 aBiketov S00a[The use of synthetic glycoconjugates as components of the immunochromatographic test for rapid serological diagnosis of leprosy.]  a289-2930 v653 a<p>The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest). The data obtained with sera of leprosy patients, patients who have been in contact with leprosy, and healthy donors indicate that the most promising antigenic component is a BSA conjugate with two synthetic epitopes - a disaccharide derivative of PGL-1 and a branched hexasaccharide fragment of LAM. The leprosy LF serotest with such glycoconjugate demonstrated the greatest diagnostic sensitivity for main forms of leprosy - paucibacillary (PB) and multibacillary (MB).</p>  a0869-2084