02461nas a2200289   4500000000100000008004100001260001200042653001400054653002300068653001900091653001900110653001200129653002300141100001400164700001000178700001200188700001300200700001400213700001300227700001300240245011200253856008000365300000900445490000700454520169600461022001402157       2020                            d  c01/202010acytokines10agene polymorphisms10ahelper T-cells10ainterleukin-1010aleprosy10aregulatory T-cells1 aTarique M1 aNaz H1 aSaini C1 aSuhail M1 aShankar H1 aKhanna N1 aSharma A00aAssociation of IL-10 Gene Polymorphism With IL-10 Secretion by CD4 and T Regulatory Cells in Human Leprosy.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7424005/pdf/fimmu-11-01974.pdf  a19740 v113 a<p>Leprosy is a chronic bacterial disease caused by . Cytokines are known to play vital role as a peacekeeper during inflammatory and other immunocompromised conditions such as leprosy. This study has tried to bridge the gap of information on cytokine gene polymorphisms and its potential role in the pathogenesis of leprosy. Interleukin-10 (IL-10) is an immunosuppressive cytokine, found to be elevated in leprosy that accounted for the suppression of host's immune system by regulating the functions of other immune cells. T helper cells and T regulatory (Tregs) cells are the major source of IL-10 in lepromatous leprosy patients. In this study, we have documented the association of IL-10 cytokine gene polymorphism with the disease progression. A total of 132 lepromatous leprosy patients and 120 healthy controls were analyzed for IL-10 cytokine gene polymorphisms using PCR-SSP assay and flow cytometry was used to analyze IL-10 secretion by CD4 and Tregs in various genotype of leprosy patients. The frequencies of IL-10 (-819) TT and IL-10 (-1082) GG genotypes were significantly higher in leprosy patients as compared to healthy controls. This observation advocates that these genotypes were associated with the susceptibility and development of the disease. In addition, flow cytometry analysis demonstrated an increased number of IL-10 producing CD4 and Treg cells in IL-10 (819) TT genotype compared to CT and CC genotypes. These observations were further supported by immunohistochemical studies. Therefore, we can conclude that IL-10 cytokine gene polymorphisms by affecting its production can determine the predilection and progression of leprosy in the study population.</p>  a1664-3224