02668nas a2200289   4500000000100000008004100001260001200042653000900054653002200063653001100085653002900096653001200125653002600137100001400163700001500177700001300192700001200205700001500217700001200232700001800244700002200262700001200284245011500296300001100411520194200422022001402364       2021                            d  c01/202110aCCL210aHousehold contact10aIFN-γ10aImmunological biomarkers10aleprosy10aSubclinical infection1 aQueiroz E1 aMedeiros N1 aMattos R1 aPinto B1 aCarvalho A1 aDutra W1 aFélix-Lana F1 aCorrea-Oliveira R1 aGomes J00aCCL2 and IFN-γ serum levels as biomarker for subclinical infection in household contacts of leprosy patients.  a1047253 a<p>Leprosy, also known as Hansen's disease, is a long-term infection by the bacteria Mycobacterium leprae, and actually still persists as a serious public health problem. The clinical parameters are used for diagnosis, however, some studies have indicated the selection of a set of biomarkers of subclinical infection, both serological and cellular, that allow the early diagnosis. Some cytokines and chemokines have been differentially expressed in index cases (paucibacillary and multibacillary patients) and household contacts (HHC), and may present a potential biomarker of M. leprae subclinical infection. Thus, the aim of this study was to analyze the variations in the profile of cytokines and chemokines, longitudinally, between index cases and their household contacts with a view to identifying possible biomarkers with differential expression, which may guide the early subclinical infection in household contacts. A longitudinal study was carried out between 2014 and 2015. The serum levels of the cytokines and chemokines were measured in all patient samples by CBA (Cytometric Bead Array). We observed a reduction of IL-4 and IL-17 expression of HHC group in the second evaluation (T1), as also a reduction of IL-17 in MB. We observed increased expression of IL-2 in PB patients as well. HHC, PB and MB showed a similar reduction profile of the chemokines CXCL8, CXCL9 and CXCL10 from T0 to T1. Interestingly, only serological levels of CCL2 are increased after a follow-up of HHC group, and this group, but not PB and MB patients, showed a significant association and a negative correlation between CCL2 and IFN-γ. The present study showed for the first time a similarity in the immunological scenario between HHC, PB and MB patients. In addition, this work highlights CCL2 chemokine in association with IFN-γ as possible biomarkers of subclinical infection of HHC, as also a parameter of early infection monitoring.</p>  a1096-1208