02295nas a2200265 4500000000100000008004100001260001000042100001200052700001100064700001200075700001200087700001200099700001200111700001200123700001600135700001800151700001300169700001500182245007300197856012200270300001200392490000700404520160400411022001402015 2020 d bLepra1 aDubey A1 aGaur R1 aArela N1 aSingh V1 aArora M1 aSagar H1 aKamal R1 aNatarajan M1 aBhagyawant SS1 aPatil SA1 aMohanty KK00aSoluble mediators of immune significance in sera of leprosy patients uhttps://leprosyreview.org/admin/public/article_shell/uploads/article_files/Lepra/LEPROSY/91/4/lr2020021/lr2020021.pdf a403-4120 v913 aBackground:
The disease leprosy is categorized by a wide spectrum of clinical forms imposed by the patient’s immune responses to the organism, Mycobacterium leprae. Soluble receptors and soluble mediators are important for regulation of immune responses.

Objectives:
As leprosy reactions are known immune inflammatory reactions during the disease process, the aim of the study was to screen nitrite, IL-6 cytokine and sTLR2 in sera samples to identify circulating biomarkers associated with leprosy and T1R and T2R among patients recruited from the Northern part of India.

Methods:
Leprosy patients recruited in our Institute were included in this study. We analysed the level of nitrite, soluble TLR2 and IL-6 in sera of leprosy patients, healthy controls and healthy household contacts by colorimetric assay and ELISA respectively.

Results:
The level of serum nitrite was observed to be higher in tuberculoid leprosy patients compared to lepromatous leprosy patients (p = 0.006). The level was also higher in healthy contacts of leprosy patients compared to lepromatous leprosy patients (p = 0.008). The sTLR2 level was significantly higher in healthy controls than leprosy patients (p = 0.002) and their healthy contacts (p = 0.001). The level of IL-6 in serum was significantly higher in leprosy patients than in healthy controls (p = 0.001).

Conclusion:
Nitrite, IL-6 cytokine and sTLR2 are shown to be potential circulating biomarkers associated with leprosy among patients recruited in Northern India. a2162-8807