01972nas a2200217 4500000000100000008004100001260001200042100001200054700001300066700001300079700001300092700001400105700001200119700001200131245012900143856009000272300000900362490000700371520136200378022001401740 2021 d c02/20211 aBayal N1 aNagpal S1 aHaque MM1 aPatole M1 aShouche Y1 aMande S1 aMande S00aStructural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India. uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870967/pdf/41598_2020_Article_80533.pdf a32940 v113 a
Although skin is the primary affected organ in Leprosy, the role of the skin microbiome in its pathogenesis is not well understood. Recent reports have shown that skin of leprosy patients (LP) harbours perturbed microbiota which grants inflammation and disease progression. Herein, we present the results of nested Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) which was initially performed for investigating the diversity of bacterial communities from lesional skin (LS) and non-lesional skin (NLS) sites of LP (n = 11). Further, we performed comprehensive analysis of 16S rRNA profiles corresponding to skin samples from participants (n = 90) located in two geographical locations i.e. Hyderabad and Miraj in India. The genus Staphylococcus was observed to be one of the representative bacteria characterizing healthy controls (HC; n = 30), which in contrast was underrepresented in skin microbiota of LP. Taxa affiliated to phyla Firmicutes and Proteobacteria were found to be signatures of HC and LS, respectively. Observed diversity level changes, shifts in core microbiota, and community network structure support the evident dysbiosis in normal skin microbiota due to leprosy. Insights obtained indicate the need for exploring skin microbiota modulation as a potential therapeutic option for leprosy.
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