02996nas a2200253   4500000000100000008004100001260001200042653001400054653003000068653001200098653004300110653004300153100001200196700001300208700001300221700001200234700001400246245013700260856008100397300001200478490000700490520223100497022001402728       2021                            d  c01/202110adiagnosis10ahistopathology/microscopy10aleprosy10aloop-mediated isothermal amplification10aquantitative polymerase chain reaction1 aJoshi S1 aSharma V1 aRamesh V1 aSingh R1 aSalotra P00aDevelopment of a novel loop-mediated isothermal amplification assay for rapid detection of Mycobacterium leprae in clinical samples.  uhttps://ijdvl.com/view-pdf/?article=2a07646a4b6e37f6f817ef285445f3c7JTMgZoY=  a491-4970 v873 a<p><b>BACKGROUND: </b>Sensitive and definitive diagnostic tests are required for timely treatment of leprosy and to control its transmission.</p><p><b>AIM: </b>In the present study, we report the development of loop-mediated isothermal amplification assay using six primers targeting the RLEP gene sequence uniquely present in Mycobacterium leprae.</p><p><b>METHODS: </b>Tissue punch samples (n = 50) and slit aspirates (n = 50) from confirmed cases of leprosy (M. leprae positive by quantitative polymerase chain reaction), reporting at the Department of Dermatology, Safdarjung Hospital, New Delhi, were analyzed using newly developed closed tube loop-mediated isothermal amplification assay. The sensitivity and specificity; positive predictive value, negative predictive value and accuracy were calculated using MedCalc statistical software.</p><p><b>RESULTS: </b>The loop-mediated isothermal amplification assay specifically amplified M. leprae genomic DNA with an analytical sensitivity of 100 fg. About 47 Out of the 50 quantitative polymerase chain reactions confirmed M. leprae positive tissue samples, 47 were positive by loop-mediated isothermal amplification assay (sensitivity 94%; 95% confidence interval 83.5%-98.8%) while only 31/50 were positive by histopathology (sensitivity 62%; 95% confidence interval 47.2%-75.4%) . Using slit aspirate samples of these 50 patients, 42 were positive by both quantitative polymerase chain reaction and loop-mediated isothermal amplification assay (sensitivity 84%; 95% confidence interval 70.9%-92.8%) while only 23/50 (sensitivity 46%; 95% confidence interval 31.8%-60.7%) were positive by microscopy.</p><p><b>LIMITATIONS: </b>In the present study, the leprosy patient cohort was not uniform, as it comprised a lower number of paucibacillary cases (22%) compared to multibacillary (78%) cases.</p><p><b>CONCLUSION: </b>Loop-mediated isothermal amplification assay established here provides a rapid and accurate diagnostic test for leprosy in terms of sensitivity and specificity. The assay is simple to perform in comparison with other molecular techniques (polymerase chain reaction/quantitative polymerase chain reaction) and has potential for field applicability.</p>  a0973-3922