02295nas a2200361   4500000000100000008004100001260001200042653003800054653002600092653001200118653001800130653001800148653001100166100001500177700001500192700001400207700001400221700001500235700001400250700001800264700001300282700002400295700001300319700001600332700001200348700002000360245016900380856008200549300000800631490000700639520127300646022001401919       2021                            d  c11/202110aEnzyme-linked immunosorbent assay10aImmunoassay protocols10aleprosy10aP. gingivalis10aPeriodontitis10aSaliva1 aCalheira M1 aTrindade S1 aFalcão M1 aBarbosa L1 aCarvalho G1 aMachado P1 aGomes-Filho I1 aCampos E1 ade Carvalho-Filho P1 aXavier M1 ade Farias A1 aFilho J1 aPassos-Soares J00aImmunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy.  uhttps://amb-express.springeropen.com/track/pdf/10.1186/s13568-021-01312-7.pdf  a1520 v113 a<p>Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relationship. Therefore, the objective of this study was to standardize an immunoassay to measure IgA specific to P. gingivalis antigens in the saliva of individuals with leprosy. An ELISA checkerboard titration was performed. A validation test involving 53 individuals with leprosy, 24 with and 19 without periodontitis, was conducted and a ROC curve constructed to calculate sensitivity and specificity. The coefficient of the optical densities was 2.21 and 2.66 for P. gingivalis crude extract and the recombinant protein HmuY, respectively. Sensitivity and specificity for the P. gingivalis crude extract were 66.7% and 73.7%, respectively, and for HmuY, were 62.5% and 52.6%, respectively. Specific recognition of P. gingivalis occurred predominantly in individuals with periodontitis, which validates the use of this test for studying periodontitis in individuals with leprosy.Trial registration&nbsp;CAEE 64476117.3.0000.0049, 21/07/2017, retrospectively registered.</p>
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