02179nas a2200433   4500000000100000008004100001260001300042653003000055653001500085653001000100653002400110653001600134653001500150653004400165653001100209653002400220653001800244653002500262653002600287653001600313653002400329653002500353653001800378100001500396700001800411700002300429700001600452700001200468700002000480700001400500700001600514700001300530700001300543245014700556300001000703490000700713520101100720022001401731       1990                            d  c1990 Aug10aAdministration, Cutaneous10aAdolescent10aAdult10aAntigens, Bacterial10aBCG Vaccine10aCell Count10aDose-Response Relationship, Immunologic10aHumans10aIn Vitro Techniques10aInterleukin-210aLeprosy, lepromatous10aLymphocyte Activation10aMiddle Aged10aPhytohemagglutinins10aRecombinant Proteins10aT-Lymphocytes1 aConverse P1 aOttenhoff T H1 aWork Teklemariam S1 aHancock G E1 aDietz M1 aBecx-Bleumink M1 aWondimu A1 aKiessling R1 aCohn Z A1 aKaplan G00aIntradermal recombinant interleukin 2 enhances peripheral blood T-cell responses to mitogen and antigens in patients with lepromatous leprosy.  a83-910 v323 a<p>Thirty-one patients with lepromatous leprosy received recombinant interleukin 2 (IL-2) intradermally in doses ranging from 10 to 30 micrograms. Before injection and at time intervals of 2-21 days thereafter, samples of peripheral blood mononuclear cells (PBMC) were obtained. Single or multiple injections (1-3) of IL-2 did not modify the total number of circulating lymphocytes or the number of T cells and the CD4/CD8 T-cell ratio. However, IL-2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4-8 days after intradermal IL-2. Stimulation indices of three- to sevenfold above pre-IL-2 levels were observed with the polyclonal activator phytohaemagglutinin (PHA) and enhanced thymidine incorporation occurred in the presence of antigens to which the patients were already sensitized, such as purified protein derivative and BCG. IL-2 had no effect on the unresponsive state of lepromatous leprosy patient T cells to the antigens of Mycobacterium leprae.</p>  a0300-9475