01713nas a2200253   4500000000100000008004100001260001000042653001900052100001600071700001900087700001800106700001400124700001400138700002000152700001900172700001700191700001100208245013600219856009800355300001200453490000700465520097300472022001401445       2022                            d  bLepra10aGeneral Energy1 aMuthusamy S1 aVilvanathan SA1 aDhandapany SP1 aBhagat VM1 aChadha VK1 aSivashanmugam E1 aKamalanathan A1 aLakshmanan S1 aMani M00aMolecular detection of drug resistance in clinical specimens of leprosy patients from a tertiary care leprosy centre in South India  uhttps://leprosyreview.org/admin/public/api/lepra/website/getDownload/63945627afaac14a3c48a28b  a370-3760 v933 a<p>Background</p>

<p>Leprosy is one of the neglected tropical diseases (NTD) experiencing relative stagnancy in the global number of new cases.</p>

<p>Objective</p>

<p>This study aims to find the proportion of antimicrobial resistance in the clinical specimens of leprosy patients attending a tertiary care leprosy Institute in South India.</p>

<p>Materials and methods</p>

<p>Skin biopsy specimens (31&nbsp;Nos) were tested by Polymerase chain reaction (PCR) for identification of&nbsp;M.&nbsp;leprae, and PCR followed by DNA sequencing for antimicrobial resistance (AMR) detection.</p>

<p>Results</p>

<p>All 31 specimens tested for&nbsp;M.&nbsp;leprae&nbsp;identification were positive. Resistance to dapsone was 6.5%, while rifampicin and ofloxacin showed 100% sensitivity.</p>

<p>Conclusion</p>

<p>This study warrants the need for a vigilant surveillance mechanism to monitor the trend of antimicrobial resistance in leprosy.&nbsp;</p>
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