02510nas a2200229   4500000000100000008004100001260001600042653001600058653001100074653001200085653001000097100001400107700001400121700001500135700001500150245009300165856015300258300001200411490000700423520183600430022001402266       2022                            d  bElsevier BV10aDermatology10aIL-17A10aLeprosy10aEgypt1 aFarag AGA1 aLabeeb AZ1 aGerges ANA1 aElshaib ME00aInterleukin-17A in Egyptian leprosy patients: a clinical, genetic, and biochemical study  uhttps://www.sciencedirect.com/science/article/pii/S0365059622002008/pdfft?md5=729ddf70b17cd491014ea1e3cffa6f0c&pid=1-s2.0-S0365059622002008-main.pdf  a735-7410 v973 a<p id="sect0010"><strong>Background:&nbsp;</strong>Leprosy represents a long-term communicable disease resulting from&nbsp;<em>Mycobacterium leprae</em>&nbsp;infection. IL-17A is one of the pro-inflammatory cytokines that protects humans against many fungal and bacterial pathogens.</p>

<p id="sect0015"><strong>Objective:&nbsp;</strong>To investigate IL-17A (rs2275913) gene polymorphism and its circulating level in leprosy patients, and to correlate the detected results with different clinical aspects of leprosy in the investigated patients.</p>

<p id="sect0020"><strong>Methods:&nbsp;</strong>60 patients with leprosy, and 29 age and sex-matched volunteers were investigated for IL-17A serum level and IL-17A single nucleotide polymorphism (SNP) by ELISA and RFLP-PCR respectively.</p>

<p id="sect0025"><strong>Results:&nbsp;</strong>IL-17A serum level was significantly higher in leprosy patients than in controls (p&nbsp;=&nbsp;0.034), and in TL than LL (p&nbsp;=&nbsp;0.017). IL-17A (rs2275913 A/G) G allele and GG genotype were associated significantly with LL (p&nbsp;=&nbsp;0.005and 0.001 respectively). IL-17A (rs2275913 A/G) AG genotype carriers demonstrated the highest IL-17A serum levels; however, its lowest levels were found in IL-17A (rs2275913 A/G) AA genotype carriers (p&nbsp;=&nbsp;0.005). Grade 2 disability (p&nbsp;=&nbsp;0.030) and positive slit skin smear (SSS) (p&nbsp;=&nbsp;0.005) were significantly associated with IL-17A (rs2275913 A/G) GG genotype.</p>

<p id="sect0030"><strong>Study limitations:&nbsp;</strong>The small number of studied subjects.</p>

<p id="sect0035"><strong>Conclusions:&nbsp;</strong>IL -17A may have a pivotal role in leprosy pathogenesis. IL-17A (rs2275913) GG genotype plus G allele might be related to the development of LL in the Egyptian population.</p>
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