02273nas a2200277   4500000000100000008004100001260001200042653001500054653001700069653002500086653001800111100001100129700001400140700001300154700001500167700001300182700001500195700001400210700001400224245010100238856008400339300000800423490000700431520154300438022001401981       2023                            d  c10/202310aChaperonin10aIgG antibody10aLepromatous patients10aPhage display1 aLima M1 aCorrêa M1 aMoraes E1 aOliveira J1 aSantos P1 ade Souza A1 aGoulart I1 aGoulart L00aHSP60 mimetic peptides from Mycobacterium leprae as new antigens for immunodiagnosis of Leprosy.  uhttps://amb-express.springeropen.com/counter/pdf/10.1186/s13568-023-01625-9.pdf  a1200 v133 a<p>The early diagnosis of leprosy serves as an important tool to reduce the incidence of this disease in the world. Phage display (PD) technology can be used for mapping new antigens to the development of immunodiagnostic platforms. Our objective was to identify peptides that mimic Mycobacterium leprae proteins as serological markers using phage display technology. The phages were obtained in the biopanning using negative and positive serum from household contacts and leprosy patients, respectively. Then, the peptides were synthesized and validated in silico and in vitro for detection of IgG from patients and contacts. To characterize the native protein of M. leprae, scFv antibodies were selected against the synthetic peptides by PD. The scFv binding protein was obtained by immunocapture and confirmed using mass spectrometry. We selected two phase-fused peptides, MPML12 and MPML14, which mimic the HSP60 protein from M. leprae. The peptides MPML12 and MPML14 obtained 100% and 92.85% positivity in lepromatous patients. MPML12 and MPM14 detect IgG, especially in the multibacillary forms. The MPML12 and MPML14 peptides had positivity of 11.1% and 16.6% in household contacts, respectively. There was no cross-reaction in patient's samples with visceral leishmaniasis, tuberculosis and other mycobacteriosis for both peptides. Given these results and the easy obtainment of mimetic antigens, our peptides are promising markers for application in the diagnosis of leprosy, especially in endemic and hyperendemic regions.</p>
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