02900nas a2200265 4500000000100000008004100001260002300042653001600065653001200081653002200093653002400115653001500139653002200154100001100176700001100187700001200198700001000210700001100220245012400231856007800355300001400433490001400447520215900461022001402620 2023 d bInforma UK Limited10aDermatology10aleprosy10alower-limb ulcers10a16S rDNA sequencing10ametabolome10askin microecology1 aWang J1 aWang B1 aLiang C1 aJin C1 aShen H00aAnalysis of Lower-Limb Ulcers in Participants with Leprosy Sequelae Using Metabolomics and 16S Ribosomal DNA Sequencing uhttps://www.tandfonline.com/doi/epdf/10.2147/CCID.S441000?needAccess=true a3465-34800 vVolume 163 a

Purpose: This study investigated microbiome and metabolome differences between ulcerated tissues and normal skin from the lower limbs of participants with leprosy.

Patients and Methods: Ulcerated tissues and surrounding normal skin were collected from the lower limbs of 28 participants with leprosy who had been cured. The 16S ribosomal DNA sequencing analysis of the samples was conducted with the Illumina NovaSeq platform to analyze the community structure and diversity of microorganisms on the skin surface, followed by non-targeted metabolomic analysis with LC-MS technology. Next, differential metabolites were statistically screened, followed by metabolic pathway analysis. The Spearman method was used to analyze the correlation between differential microbiota and differential metabolites.

Results: Compared to normal skin, ulcerated tissues showed a decrease in microbial α diversity (species richness, homogeneity, and sequencing depth), without significant differences (observed species, Chao1, Shannon, Simpson, and Pielou’s evenness index; P > 0.05). Conversely, Jaccard distance demonstrated that sample β-diversity exhibited a certain degree of clustering (P < 0.05), with significant differences between the two groups. The results of LEfSe analysis revealed that compared to the normal skin, the ulcerated tissues had significantly decreased microbial abundance of Flavobacteriaceae, Flavobacteriales, Lachnospiraceae, Lachnospirales, Enterobacterales, Acinetobacter, and Moraxellaceae, which might be associated with the ulcerative state. The Spearman correlation analysis suggested a strong correlation between skin metabolome and skin microbiome.

Conclusion: For participants with leprosy sequelae, skin microecology and metabolites are disturbed and species diversity and homogeneity are reduced in lower-limb ulcers, and the types of skin metabolites are dependent on the microbiota.

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