02134nas a2200253   4500000000100000008004100001260001200042653001400054653001400068653002000082653001200102653001500114653001400129100001400143700001600157700001100173700001700184700001400201700001800215245010200233856015300335520137800488022001401866       2024                            d  c01/202410aApoptosis10aAutophagy10aBacillary index10aleprosy10aPhenotypes10aMicroRNAs1 aSantana N1 ade Farias L1 aLago T1 aLeal-Calvo T1 aMachado P1 aCastellucci L00aMicroRNAs correlate with bacillary index and genes associated to cell death processes in leprosy.  uhttps://www.sciencedirect.com/science/article/pii/S1286457924000200/pdfft?md5=6fbd0911f7e6bacfda60599a27cfcefb&pid=1-s2.0-S1286457924000200-main.pdf3 a<p>Mycobacterium leprae infects skin and peripheral nerves causing a broad of clinical forms. MicroRNAs (miRNAs) control immune mechanisms such as apoptosis, autophagy as well as to target genes leading to abnormal proliferation, metastasis, and invasion of cells. Herein we evaluated miRNAs expression for leprosy phenotypes in biopsies obtained from patients with and without reactions. We also correlated those miRNAs with both, bacillary index (BI) and genes involved in the micobacteria elimination process. Our results show a significant increase in the miR-125a-3p expression in paucibacillary (PB) patients vs multibacillary (MB) subjects (p = 0.007) and vs reversal reactions (RR) (p = 0.005), respectively. Likewise, there was a higher expression of miR-125a-3p in patients with erythema nodosum leprosum (ENL) vs MB without reactions (p = 0.002). Furthermore, there was a positive correlation between miR-125a-3p, miR-146b-5p and miR-132-5p expression and BI in patients with RR and ENL. These miRNAS were also correlated with genes such as ATG12 (miR-125a-3p), TNFRSF10A (miR-146b-5p), PARK2, CFLAR and STX7 (miR-132-5p). All together we underpin a role for these miRNAs in leprosy pathogenesis, implicating mechanisms such as apoptosis and autophagy in skin. The miR-125a-3p might have a distinct role associated with PB phenotype and ENL in MB patients.</p>
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