01967nas a2200229   4500000000100000008004100001260001200042100001100054700001000065700001000075700001000085700000900095700001000104700001000114700001200124245008600136856009800222300000900320490000700329520138700336022001401723       2024                            d  c01/20241 aZhou Q1 aShi P1 aShi W1 aGao J1 aWu Y1 aWan J1 aYan L1 aZheng Y00aIdentification of potential biomarkers of leprosy: A study based on GEO datasets.  uhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0302753&type=printable  a1-150 v193 a<p>Leprosy has a high rate of cripplehood and lacks available early effective diagnosis methods for prevention and treatment, thus novel effective molecule markers are urgently required. In this study, we conducted bioinformatics analysis with leprosy and normal samples acquired from the GEO database(GSE84893, GSE74481, GSE17763, GSE16844 and GSE443). Through WGCNA analysis, 85 hub genes were screened(GS &gt; 0.7 and MM &gt; 0.8). Through DEG analysis, 82 up-regulated and 3 down-regulated genes were screened(|Log2FC| &gt; 3 and FDR &lt; 0.05). Then 49 intersection genes were considered as crucial and subjected to GO annotation, KEGG pathway and PPI analysis to determine the biological significance in the pathogenesis of leprosy. Finally, we identified a gene-pathway network, suggesting ITK, CD48, IL2RG, CCR5, FGR, JAK3, STAT1, LCK, PTPRC, CXCR4 can be used as biomarkers and these genes are active in 6 immune system pathways, including Chemokine signaling pathway, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Natural killer cell mediated cytotoxicity and Leukocyte transendothelial migration. We identified 10 crucial gene markers and related important pathways that acted as essential components in the etiology of leprosy. Our study provides potential targets for diagnostic biomarkers and therapy of leprosy.</p>
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