This paper deals with studies on a number of oxidative enzymes in Mycobacterium leprae separated directly from human leprosy nodules. In an investigation such as this, certain limitations are inherent: ( 1 ) the microorganisms have to be separated from the infected tissue in pure form suitable for metabolic studies; (2) only those enzymes can be studied for which technics applicable to small quantities or material are available. Study of M. lepramnurium has become a model system for investigations relating to human leprosy. Since these organisms have also not been cultivated in inert media, they are isolated directly from infected tissues of rats or mice. Gray (9), and Hanks (10), working with whole cells of M. lepraemurium separated from testicular lepromas of rats, could not obtain utilization of 50 different substrates, including various tissue extracts. Ito and Sonoda (.17) demonstrated increase in oxygen-uptake of M. lepraemul'ium with heated extracts of rat testes and testicular lepromas. In cellnfree extracts of M. lepraemurium, Kusaka (18) observed succinate oxidase and glucose-6-phosphate dehydrogenase, which were absent from the whole cells. Mori et al. (19) demonstrated diaphorase I and II and malate dehydrogenase in extracts prepared from lyophilized cells of M. le}J - raemul'ium.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 -https://www.ncbi.nlm.nih.gov/pubmed/4290018
DA - 01/1967 IS - 1 J2 - Int J Lepr Other Mycobact Dis LA - ENG M3 - Article N2 -This paper deals with studies on a number of oxidative enzymes in Mycobacterium leprae separated directly from human leprosy nodules. In an investigation such as this, certain limitations are inherent: ( 1 ) the microorganisms have to be separated from the infected tissue in pure form suitable for metabolic studies; (2) only those enzymes can be studied for which technics applicable to small quantities or material are available. Study of M. lepramnurium has become a model system for investigations relating to human leprosy. Since these organisms have also not been cultivated in inert media, they are isolated directly from infected tissues of rats or mice. Gray (9), and Hanks (10), working with whole cells of M. lepraemurium separated from testicular lepromas of rats, could not obtain utilization of 50 different substrates, including various tissue extracts. Ito and Sonoda (.17) demonstrated increase in oxygen-uptake of M. lepraemul'ium with heated extracts of rat testes and testicular lepromas. In cellnfree extracts of M. lepraemurium, Kusaka (18) observed succinate oxidase and glucose-6-phosphate dehydrogenase, which were absent from the whole cells. Mori et al. (19) demonstrated diaphorase I and II and malate dehydrogenase in extracts prepared from lyophilized cells of M. le}J - raemul'ium.
PY - 1967 SP - 34 EP - 41 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Metabolism of Mycobacterium leprae separated from human leprosy nodules. UR - http://ila.ilsl.br/pdfs/v35n1a05.pdf VL - 35 SN - 0148-916X ER -