TY - JOUR KW - Animals KW - Epithelium KW - Fluorescein-5-isothiocyanate KW - Fluoresceins KW - Granuloma KW - Guinea Pigs KW - Haptens KW - Hypersensitivity, Delayed KW - Immunity, Cellular KW - leprosy KW - Lymph Nodes KW - Mycobacterium leprae KW - Phagocytes KW - Thiocyanates KW - Tuberculin AU - Verghese S AU - Curtis J AU - Turk J L AB -

Fluorescein isothiocyanate (FITC)-conjugated Mycobacterium leprae (FITC-M. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. At 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with FITC-M. leprae. At 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with haptenated M. leprae compared with those injected with unconjugated M. leprae. At 5 weeks, there was also a significant increase in delayed type hypersensitivity responses to 25 micrograms purified protein derivative. Histologically, epithelioid cell granulomas were seen in these lymph nodes as early as 2 weeks when FITC-M. leprae was used as the source of antigen. Enhancement in the immunogenicity of M. leprae by conjugation with FITC has been postulated.

BT - Cellular immunology C1 - http://www.ncbi.nlm.nih.gov/pubmed/3109743?dopt=Abstract DA - 1987 Jul DO - 10.1016/0008-8749(87)90239-5 IS - 2 J2 - Cell. Immunol. LA - eng N2 -

Fluorescein isothiocyanate (FITC)-conjugated Mycobacterium leprae (FITC-M. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. At 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with FITC-M. leprae. At 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with haptenated M. leprae compared with those injected with unconjugated M. leprae. At 5 weeks, there was also a significant increase in delayed type hypersensitivity responses to 25 micrograms purified protein derivative. Histologically, epithelioid cell granulomas were seen in these lymph nodes as early as 2 weeks when FITC-M. leprae was used as the source of antigen. Enhancement in the immunogenicity of M. leprae by conjugation with FITC has been postulated.

PY - 1987 SP - 307 EP - 16 T2 - Cellular immunology TI - Epithelioid cell granuloma induction in the guinea pig by haptenated Mycobacterium leprae. VL - 107 SN - 0008-8749 ER -