TY - JOUR KW - Amino Acid Sequence KW - Antibodies, Monoclonal KW - Antigens, Bacterial KW - Bacterial Proteins KW - Chromatography, Affinity KW - Humans KW - Hypersensitivity, Delayed KW - Molecular Weight KW - Mycobacterium bovis KW - Mycobacterium leprae KW - T-Lymphocytes KW - Tuberculin KW - Tuberculosis AU - Britton W J AU - Hellqvist L AU - Basten A AU - Inglis A S AB -

The protein antigens from Mycobacterium bovis (BCG), M. tuberculosis, and M. leprae share a number of common determinants. We have used a murine mAb (L7) recognizing such a determinant on a protein of Mr 70,000 to purify this antigen from M. bovis sonicate by affinity chromatography. Enrichment of the protein in column eluates was confirmed by immunoblotting and in antigen inhibition assays. After radiolabelling with 125I, the protein could be immunoprecipitated with human lepromatous leprosy sera. Stimulation of peripheral blood mononuclear cells from BCG-vaccinated and naturally mantoux-positive individuals induced proliferation and IFN-gamma secretion, while intradermal injection of purified antigen into the same subjects resulted in a delayed-type hypersensitivity reaction. Thus, the 70,000 molecule carried epitopes capable of reacting with B cells, and eliciting a potentially protective T cell response. The first 15 N-terminal residues were sequenced using a gas-phase sequenator.

BT - The Journal of experimental medicine C1 - http://www.ncbi.nlm.nih.gov/pubmed/3528377?dopt=Abstract DA - 1986 Sep 01 DO - 10.1084/jem.164.3.695 IS - 3 J2 - J. Exp. Med. LA - eng N2 -

The protein antigens from Mycobacterium bovis (BCG), M. tuberculosis, and M. leprae share a number of common determinants. We have used a murine mAb (L7) recognizing such a determinant on a protein of Mr 70,000 to purify this antigen from M. bovis sonicate by affinity chromatography. Enrichment of the protein in column eluates was confirmed by immunoblotting and in antigen inhibition assays. After radiolabelling with 125I, the protein could be immunoprecipitated with human lepromatous leprosy sera. Stimulation of peripheral blood mononuclear cells from BCG-vaccinated and naturally mantoux-positive individuals induced proliferation and IFN-gamma secretion, while intradermal injection of purified antigen into the same subjects resulted in a delayed-type hypersensitivity reaction. Thus, the 70,000 molecule carried epitopes capable of reacting with B cells, and eliciting a potentially protective T cell response. The first 15 N-terminal residues were sequenced using a gas-phase sequenator.

PY - 1986 SP - 695 EP - 708 T2 - The Journal of experimental medicine TI - Immunoreactivity of a 70 kD protein purified from Mycobacterium bovis Bacillus Calmette-Guerin by monoclonal antibody affinity chromatography. VL - 164 SN - 0022-1007 ER -